Cinnamaldehyde decreases interleukin-1beta induced PGE2 production by down-regulation of mPGES-1 and COX-2 expression in mouse macrophage RAW264.7 cells.
- Author:
Changbin ZHANG
1
;
Canghai LI
;
Feng SUI
;
Yin LU
;
Lanfang LI
;
Shuying GUO
;
Na YANG
;
Daitao GENG
;
Tingliang JIANG
Author Information
1. Nanjing University of Chinese Medicine, Nanjing 210029, China.
- Publication Type:Journal Article
- MeSH:
Acrolein;
analogs & derivatives;
pharmacology;
Animals;
Blotting, Western;
Cell Line;
Dinoprostone;
metabolism;
Interleukin-1beta;
pharmacology;
Intramolecular Oxidoreductases;
metabolism;
Macrophages;
drug effects;
metabolism;
Mice;
Prostaglandin-E Synthases;
Real-Time Polymerase Chain Reaction
- From:
China Journal of Chinese Materia Medica
2012;37(9):1274-1278
- CountryChina
- Language:Chinese
-
Abstract:
Cinnamaldehyde was shown to have significant anti-inflammatory and anti-pyretic actions in studies from both others' and our lab. Prostaglandin E2 (PGE2) plays a key role in generation of these pathological states, while PGE, synthase-1 (mPGES-1) is one of crucial biological elements in the process of PGE2 production. And as a downstream inducible terminal prostaglandin synthase of COX-2, mPGES-1 is now regarded as a more promising novel drug target than COX-2 and is attracting more and more attention from both academia and pharmaceutical industry. The purpose of present study was to further investigate the anti-inflammatory and antipyretic molecular mechanisms of cinnamaldehyde based on the mouse macrophage cell line RAW264. 7 in vitro. The PGE2 was identified by using the method of enzyme-linked immunosorbent assay (ELISA) and the expression of COX-2 and mPGES-1 at mRNA and protein levels was detected by the Real-time PCR and Western blotting methods respectively. The experimental results suggested that cinnamaldehyde could evidently reverse the increased production of PGE2induced by IL-1beta. Moreover, the up-regulated expression levels of mPGES-1 and COX-2 were significatly decreased. Together, these results provide compelling evidence that the down-regulated actions to both the production of PGE2 as well as the expression of mPGES-I might account for, at least in part, the anti-inflammatory and anti-pyretic effects of cinnamaldehyde.
