Study on Th1/Th2 transcriptional regulatory mechanism of san'ao tang in bronchial asthmatic rats.
- Author:
Hui CHEN
1
;
Rong MA
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Asthma; drug therapy; metabolism; Disease Models, Animal; Drugs, Chinese Herbal; therapeutic use; Male; Rats; Rats, Sprague-Dawley; Th1 Cells; drug effects; metabolism; Th2 Cells; drug effects; metabolism
- From: China Journal of Chinese Materia Medica 2012;37(9):1324-1326
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the regulatory effect of San'ao Tang on Thl/Th2 cells and its interference on transcription factor GATA-3 and T-bet-mRNA in asthmatic rats.
METHODRat asthma model was established by abdominal injection and aerosol inhalation of OVA. The content of cytokines IL-4 and IFN-gamma in alveolus lavage fluid of asthmatic rats was detected before and after the oral administration with large, medium, low dose of San'ao Tang and dexamethasone. T-bet and GATA-3 mRNA expressions in lung tissues were determined by RT-PCR.
RESULTCompared with other groups, the medium-dose group showed a notable decrease in IL-4 in rat BALF and an increasing IFN-gamma. Its IL-4/IFN-gamma is significant lower than the asthma group, with expressions of lung tissue GATA-3 and transcription factor T-bet-mRNA in line with changes in IL-4 and IFN-gamma.
CONCLUSIONSan'ao Tang may have a inhibitory effect on airway inflammation of asthmatic rats by regulating Th1/Th2 transfer factors.