Alpha-glucosidase inhibitory active constituents contained in nutshell of Trapa acornis.
- Author:
Baiquan CHEN
1
;
Qian ZHANG
;
Wei WANG
;
Huan HUANG
;
Wenyi KANG
Author Information
- Publication Type:Journal Article
- MeSH: Enzyme Inhibitors; chemistry; Glycoside Hydrolase Inhibitors; Lythraceae; chemistry
- From: China Journal of Chinese Materia Medica 2012;37(10):1408-1411
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study chemical constituents in nutshell of Trapa acornis and in vitro inhibitory activity against alpha-glucosidase.
METHODEtOAC and n-butanol extractive fractions were separated by chromatography and their structures were identified by multiple spectroscopic techniques.
RESULTNine compounds were separated, they were 4,23,24-trimethylcholest-22-en-3-ol (1), stigmasterol (2), alpha-amyrin (3), (+)-nyasol (4), oleanolic acid (5), ursolic acid (6), hederagenin (7), 3,23-dihydroxy-12-ursen-28-oic acid (8) and beta-daucosterol (9). Total extracts from T. acornis nutshells, petroleum ether fractions, acetic ether fractions and normal butanol fractions showed inhibitory activity against alpha-glucosidase. Compound 5 (IC50 2.88 mg x L(-1)) and 6 (IC50 4.42 mg L(-1)) showed stronger inhibitory activity against alpha-glucosidase.
CONCLUSIONAll compounds except compound 2 were separated from the genus for the first time, and compound 1-9 were separated from this plant for the first time.
