Comparative study on tumor cell apoptosis in vitro induced by extracts of Stellera chamaejasme.
- Author:
Xiaoni LIU
1
;
Yujie LI
;
Qing YANG
;
Ying CHEN
;
Xiaogang WENG
;
Yiwei WANG
;
Xiaoxin ZHU
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; drug effects; Caspases; metabolism; Cell Line, Tumor; Humans; Neoplasms; chemistry; drug therapy; pathology; Plant Extracts; pharmacology; Receptors, TNF-Related Apoptosis-Inducing Ligand; analysis; Thymelaeaceae; chemistry; Tumor Necrosis Factor-alpha; analysis; fas Receptor; analysis
- From: China Journal of Chinese Materia Medica 2012;37(10):1440-1444
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo in vitro compare the induction of extracts of Stellera chamaejasme ESC, ESC-1 and ESC-2 on NCI-H157 cell apoptotic.
METHODThe apoptosis rate was inspected by flow cytometry; caspase-3, 8, 9 activities was measured by spectrophotometry. Fas, Fas-L, TNF-alpha, Trail-R, Cyto-C, Smac/diablo protein expressions of apoptosis pathway was observed by Elisa method.
RESULTCompared with the control group, ESC, ESC-1, ESC-2 can significantly improve the apoptosis rate of NCI-H157 cell. ESC significantly improved cells caspase-3, 8 activity, ESC-2 can significantly improve the activity of caspase-3, 8, 9. ESC, ESC-1, ESC-2 significantly increased Fas expression and ESC significantly increased Fas/Fas-L ratio. ESC, ESC-1, ESC-2 significantly increased TNF-alpha protein expression. ESC-1 significantly lowered TRAIL-R expression. ESC, ESC-1, ESC-2 had no significant effect on Cyto-C. ESC-1, ESC-2 significantly reduced Smac protein expression.
CONCLUSIONThe apoptotic effect induced by ESCs may be related to the regulation of death receptor pathway proteins. Induction mechanisms of ESCs were so complicated that it may have a two-way regulatory effect. Its induction in apoptosis is a result from comprehensive regulation and control.
