Pollen morphological characteristics, viability test and storage of endangered medicinal plant Atractylodes lancea.
- Author:
Lin LI
1
;
Wei GU
;
Jianguo CHAO
;
Jie GAO
;
Juanjuan ZHOU
;
Xiuyuan SHEN
Author Information
- Publication Type:Journal Article
- MeSH: Atractylodes; drug effects; growth & development; physiology; ultrastructure; Endangered Species; Germination; drug effects; Microscopy, Electron, Scanning; Plants, Medicinal; drug effects; growth & development; physiology; ultrastructure; Pollen; drug effects; growth & development; physiology; ultrastructure; Polyethylene Glycols; pharmacology
- From: China Journal of Chinese Materia Medica 2012;37(11):1546-1549
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the pollen morphological characteristics, viability test and storage character of the endangered plant Atractylodes lancea.
METHODPollen grains morphologies of A. lancea were observed by scanning electron microscope. The optimum culture medium and viability determination methods were screened out by liquid culture and dyeing methods, and then the pollen germination capacities in different storage conditions were detected.
RESULTThe pollen grains are quasi-spherical, with tricolpate and spinous sculpture. The optimal culture medium was ME3 + 16% PEG4000 + 10% sucrose, in which the pollen germination capacity reached to 62.1%, while the other three dyeing methods were not able to be applied to detecting the pollen viability of A. lancea. The low storage temperature could significantly prolong the storage time of pollen of A. lancea. At -80 degrees C, pollen viability could be maintained for 60 days.
CONCLUSIONLiquid culture method is suitable for the determination of pollen germination of A. lancea, and the rate of pollen germination is closely related to the storage time and temperature. At last, this study provides a foundation for the artificial pollination and cultivating in wildness of A. lancea.
