Genetic relationship on several medicinal plants in Marsdenia from Yunnan in ISSR marker.
- Author:
Zhibin GUAN
1
;
Lixia ZHANG
;
Meifang SONG
;
Haitao LI
;
Zhonglian ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: China; Genetic Markers; genetics; Marsdenia; classification; genetics; Medicine, Chinese Traditional; Microsatellite Repeats; genetics; Phylogeny; Plants, Medicinal; classification; genetics; Polymerase Chain Reaction
- From: China Journal of Chinese Materia Medica 2012;37(11):1550-1552
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo analyze the genetic relationship of 9 Marsdenia species from Yunnan, especially the traditional Dai medicine "Dai Bai Jie" (M. auricularis).
METHODApplying the inter-simple sequence repeats (ISSR) markers technology.
RESULTTwenty-five primers were screened out of 60 ISSR random primers and produced 391 bands totally, every primer produce 8-21 bands and the mean number was 15.6. The range of the GS (genetic similarity) value was 0.6675-0.8210. In 9 Marsdenia species, M. auricularis is a relative of M. tenacissima. M. balansae and M. officinalis have the closest genetic relationship.
CONCLUSIONIt is supported by ISSR that the M. auricularis which is sib species of M. tenacissima, and the folk medicine of Marsdenia are worthy deep investigation and study.
