Quantitative determination of sibricose A5 and sibricose A6 in Polygalae radix.
- Author:
Changqing SUN
1
;
Xiaomin PENG
;
Nailiang ZHU
;
Yanyan JIANG
;
Lili KANG
;
Xiaohui CUI
;
Xiaohong GUO
;
Renbing SHI
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; Drugs, Chinese Herbal; chemistry; Polygala; chemistry; Polysaccharides; analysis; chemistry; isolation & purification
- From: China Journal of Chinese Materia Medica 2012;37(11):1607-1609
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish the method for quantitative determination of sibricose A5 and sibricose A6 in Polygalae Radix by HPLC.
METHODThe ultrasonic extracting method was applied in sample pre-treatment. The HPLC procedure was performed on the chromatographic column of Agela Promosil C18 (4.6 mm x 250 mm, 5 microm), the mobile phase was acetonitrile-0.1% phosphoric acid water solution (10:90). The detection wavelength was 330 nm and flow velocity was 1 mL x min(-1). The column temperature was 30 degrees C.
RESULTThe method has good linearity in the ranges of 0.0087-0.0694 g x L(-1) (r = 0.9993) for sibricose A5, 0.0090-0.0723 g x L(-1) (r=0.9991) for sibricose A6. The average recoveries of sibricose A5 and sibricose A6 were 101.7%, 97.87%, with the RSD of 1.7%, 1.6%, respectively.
CONCLUSIONThe method was simple, quick accurate and reliable. It is appropriate for the quantitative determination of sibricose A5 and sibricose A6 in Polygalae Radix.
