Effect of pine pollen on kidney mitochondria DNA deletion mutation in senile mice.
- Author:
Lu YU
1
;
Chunxia SHI
Author Information
- Publication Type:Journal Article
- MeSH: Aging; drug effects; genetics; Animals; DNA, Mitochondrial; genetics; Drugs, Chinese Herbal; pharmacology; Female; Kidney; drug effects; metabolism; Male; Mice; Pinus; chemistry; Pollen; chemistry; Sequence Deletion; drug effects
- From: China Journal of Chinese Materia Medica 2012;37(11):1663-1666
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of pine pollen on Kidney Mitochondria DNA Deletion Mutation (mtDNA) in senile mice.
METHODKunming senile mice were randomly divided into the pine pollen group, and the senile control group. And a young control group was randomly selected. Mouse in the pine pollen group were orally administered with pine pollen (750 mg x kg(-1)) daily. The young control group and the senile control group were orally administered with isometric 0.9% sodium chloride injection. After 60 days, deletion mutation of mtDNA were detected by PCR technology and photodensity scan. Relative level of MDA and activity of SOD in kidney tissues were detected.
RESULTThe senile control group showed significant increase in relative level and deletion mutation of mtDNA (P < 0.05). Compared with the senile control group, the pine pollen group showed decreased depletion of kidney mtDNA (P < 0.05). Pine pollen can decrease MDA volume and increase the activity of SOD significantly (P < 0.05).
CONCLUSIONPine pollen can inhibit deletion mutation of mtDNA in senile mice, suggesting that pine pollen can reduce oxidative damage of mtDNA and protect mtDNA. Accordingly, it provides a possible mechanism of anti-aging effect of pine pollen at the molecular level.
