Preservation of germplasm of Rehmannia glutinosa in vitro.
- Author:
Xue-sen WEN
1
;
Jian-he WEI
;
Shi-lin YANG
;
Xiao-jun MA
Author Information
- Publication Type:Journal Article
- MeSH: Agar; Culture Media; Culture Techniques; Plants, Medicinal; growth & development; Rehmannia; growth & development; Temperature; Tissue Preservation; methods
- From: China Journal of Chinese Materia Medica 2003;28(1):17-20
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a method for the germplasm preservation of R. glutinosa.
METHODPlantlets of different cultivars obtained by tip culture were inoculated into test tubes with MS medium supplemented with 0.2 mg.L-1 BA and 0.02 mg.L-1 NAA, and preserved at 4-6 degrees C in dark. At the same time, different media (A. distilled water + 10 g.L-1 agar; B. 1/2 MS + 5 g.L-1 agar; C. MS + 10 g.L-1 agar; D. 1/2 MS + 0.5 BA + 0.02 NAA + 10 g.L-1 agar; E. MS + 0.5 BA + 0.02 NAA + 10 g.L-1 agar) were set to conserve plantlets of "85-5" on the same condition.
RESULT4 months later, the death rate of "Xinggeda" was 73%, "Tucheng" 60%, "85-5" 33%, and "Beijing 1" 9%. All of the plantlets of "85-5" in different media kept alive. 10 months later, most of the preserved plantlets browned and wilted except those on medium A.
CONCLUSIONThe low temperature endurance of R. glutinosa is cultivar-dependent. Medium A can preserve "85-5" for more than 10 months in vitro.
