Determination of schisandrin in shengmaiyin by HPLC.
- Author:
Xiao-feng WU
1
;
Hong CHEN
;
Zhi-ru ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: Administration, Oral; Chromatography, High Pressure Liquid; Cyclooctanes; analysis; Drug Combinations; Drugs, Chinese Herbal; administration & dosage; chemistry; isolation & purification; Lignans; analysis; Plants, Medicinal; chemistry; Polycyclic Compounds; analysis; Schisandra; chemistry
- From: China Journal of Chinese Materia Medica 2003;28(1):39-72
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine the concentration of schisandrin in Shengmaiyin with HPLC.
METHODThe sample was extracted with ethyl acetate through supersonic wave. The solution was filtrated and evaporated. The residue was resolved with methanol and determined by HPLC using PHENOMENEX C18 (4.6 mm x 250 mm, 5 microns) chromatographic column, methanol-acetonitrile-water (15:15:10) as mobile phase. The wavelength for detection was 254 nm.
RESULTThe peak of schizandrin appears on about 7.10 minutes. The standard curves of schizandrin were linear in the concentration range of 0.2-2.0 micrograms, r = 0.9996. The average recovery of schizandrin were 100.5% (RSD 2.84%).
CONCLUSIONThis method was found to be sensitive, quick and accurate for the measurement of schizandrin concentrations in Shengmaiyin.
