Determination of puerarin, daidzin and daidzein in root of Pueraria lobata of different origin by HPLC.
- Author:
Wen-shan JIN
1
;
Yu-yuan TAN
;
You-gen CHEN
;
Yan WANG
Author Information
- Publication Type:Journal Article
- MeSH: China; Chromatography, High Pressure Liquid; methods; Ecosystem; Isoflavones; analysis; Plant Roots; chemistry; Plants, Medicinal; chemistry; Pueraria; chemistry; Quality Control
- From: China Journal of Chinese Materia Medica 2003;28(1):49-51
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop an HPLC method to determine Puerarin, Daidzin and Daidzein in Pueraria lobata.
METHODThe separation was performed in a SYMMETRY C18 column with a mobile phase of methanol-1% HAC solution (gradient elution), the The detection wavelength being 250 nm.
RESULTThe average recovery was respectively 101.7%, 100.7% and 101.7% (n = 3), RSD 0.43%, 0.82% and 1.50% (n = 3) for Puerarin, Daidzin and Daidzein.
CONCLUSIONThis method is suitable for the determination of Puerarin, Daidzin and Daidzein in Pueraria lobata and its preparation.
