Study on the anti-apopotosis induced by hypoxia/hypoglycemia and reoxygenation of panax notoginseng saponins in cultured rat hippocampal neurons.
- Author:
Ling-Qun ZHU
1
;
Ji-ping FAN
;
Qi-fu HUANG
;
Su-lun SUN
;
Ying GAO
;
Yi-huai ZOU
;
Zhuang ZHANG
;
Li-yun HE
;
Hong ZHENG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; drug effects; Calcium; metabolism; Cell Hypoxia; Cells, Cultured; Fetus; Ginsenosides; isolation & purification; pharmacology; Hippocampus; cytology; Hypoglycemia; pathology; L-Lactate Dehydrogenase; metabolism; Neurons; cytology; Panax; chemistry; Plants, Medicinal; chemistry; Rats; Rats, Wistar
- From: China Journal of Chinese Materia Medica 2003;28(1):52-55
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the inhibitory effects of Panax Notoginseng Saponins(PNS) on apoptosis induced by hypoxia/hypoglycemia and reoxygenation in cultured rat hippocampal neurons.
METHODApoptosis were measured by flow cytometry, intracellular free calcium concentration([Ca2+]i) was measured with confocal laser scanning microscopy, morphological changes and neuronal necrosis were observed with fluorescence microscope, and meanwhile the leakage of lactic dehydrogenase(LDH) was measured.
RESULTHypoxia/hypoglycemia cultures for 5 hours and reoxygenation induced neuronal apoptosis and necrosis, and significantly increased neuronal [Ca2+]i and the leakage of LDH. The effects were increased with the extending time of reoxygenation. PNS has could significantly decrease the percentage of neuronal apoptosis and necrosis, and reduce neuronal [Ca2+]i and the leakage of LDH.
CONCLUSIONPNS has inhibitory effect on neuronal apoptosis. This effect might be related to its effect of decreasing intracellular free calcium concentration.
