Study on plant tissue culture of Cornus officinalis.
- Author:
Jian-ping XUE
1
;
Ai-min ZHANG
;
Yue-hui WANG
;
Wei SHENG
Author Information
- Publication Type:Journal Article
- MeSH: Cornus; growth & development; Culture Media; Culture Techniques; Plant Growth Regulators; pharmacology; Plant Roots; growth & development; Plant Stems; growth & development; Plants, Medicinal; growth & development
- From: China Journal of Chinese Materia Medica 2003;28(2):118-121
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo set up the optimums of tissue culture for Cornus officinalis.
METHODA section of stems of the fine varieties of Cornus officinalis were strictly sterilized, cultivated and geminated in medium with addition of different kinds of hormone.
RESULT AND CONCLUSIONThe WPM medium with 6-BA 2.0 mg.L-1 + ZT 0.1 mg.L-1 + NAA 0.1 mg.L-1 was the optimum medium for the multiplication of adventitious buds. The WPM medium with 6-BA 1.0 mg.L-1 + ZT 0.1 mg.L-1 + GA3 0.5 mg.L-1 + NAA 0.1 mg.L-1 was the optimum medium for cultivation in strength. The plantlet could root well in 1/2 MS medium with IBA 1.0 mg.L-1 + 6-BA 0.1 mg.L-1.