Determination of 2,3,5,4'-tetrahydroxystilbene-2-O-beta-D-glucoside in yangan oral liquid by HPLC.
- Author:
Hui-ping ZHAO
1
;
Yi-kun SUN
;
Xiao-qian ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Drug Combinations; Drugs, Chinese Herbal; administration & dosage; chemistry; isolation & purification; Glucosides; analysis; Plants, Medicinal; chemistry; Polygonum; chemistry; Quality Control; Stilbenes; analysis
- From: China Journal of Chinese Materia Medica 2003;28(2):133-176
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish the determination method of 2,3,5,4'-tetrahydroxystilbene-2-O-beta-D-glucoside in Yangan Oral Liquid.
METHOD2,3,5,4'-tetrahydroxystilbene-2-O-beta-D-glucoside was determined by HPLC on C18 column, in acetonitrile-water (14:86) solution as a mobile phase, detection wavelength as 320 nm.
RESULTThis method showed a good linear relationship. The average recovery was 99.05% and RSD was 1.31%.
CONCLUSIONThe method was simple with styong specificity and good repriducibility and can be used for quality control for Yangan Oral Liquid.
