Research on expression of somatomedin b domain of proteoglycan 4 and recombinant protein aggregation.
- Author:
Lifang WANG
;
Zhibo HAN
;
Wenhu CHEN
;
Peng DU
;
Aihua SUN
;
Ping YANG
;
Hongguang ZHAO
- Publication Type:Journal Article
- MeSH:
Glycosylation;
Protein Multimerization;
Proteoglycans;
chemistry;
Recombinant Proteins;
chemistry;
Somatomedins;
chemistry
- From:
Journal of Biomedical Engineering
2014;31(6):1319-1324
- CountryChina
- Language:Chinese
-
Abstract:
Recombinant protein SMB(PRG4) containing two Somatomedin B domains and a small amount of glycosylation of repetitive sequences of proteoglycan 4 was cloned according to PGR4 gene polymorphism. Mature purification process was established and recombinant protein SMB(PRG4), with high-level expression was purified. By using size-exclusion chromatogaraphy and dynamic light scattering, we found that the recombinant protein self-aggregate to dimeric form. Structure prediction and non-reducing electrophoresis revealed that SMB(PRG4), was a non-covalently bonded dimer.
