Inhibition of dexamethasone, indomethacin and resveratrol on matrix metalloproteinase-9 and the mechanism of inhibition.
- Author:
Yi-tang LI
1
;
Fang SHEN
;
Jin-ye BAI
;
Gui-fang CHENG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Anti-Inflammatory Agents; pharmacology; Anti-Inflammatory Agents, Non-Steroidal; pharmacology; Croton Oil; Dexamethasone; pharmacology; Ear Diseases; chemically induced; metabolism; Edema; chemically induced; metabolism; Humans; Indomethacin; pharmacology; Male; Matrix Metalloproteinase 9; metabolism; Matrix Metalloproteinase Inhibitors; Mice; Mice, Inbred ICR; Random Allocation; Stilbenes; pharmacology; U937 Cells; metabolism
- From: Acta Pharmaceutica Sinica 2003;38(7):501-504
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo investigate the expression of matrix metalloproteinase-9 (MMP-9) in mouse ears induced with croton oil and the inhibitory effect of dexamethasone, indomethacin and resveratrol on MMP-9 expression, and further explore the relationship between anti-inflammation and MMP-9 inhibition of these three medicines.
METHODSImmuno-histochemistry was used to detect the expression of MMP-9 in mouse ears. Expression of MMP-9 in U937 cells was analyzed by gelatin zymography.
RESULTSMouse ear edema induced with croton oil was inhibited significantly by dexamethasone and indomethacin at the dose of 10 mg.kg-1 and resveratrol at 50 mg.kg-1 administered subcutaneously. The inhibitory rate was 76.2% (P < 0.001), 56.7% (P < 0.001) and 36.9% (P < 0.001) respectively. The MMP-9 expression increased in mouse ears induced with croton oil and inhibited by dexamethasone, indomethacin and resveratrol at above doses. Gelatin zymography results showed that MMP-9 expression in U937 cells increased significantly after exposed to PMA at 1 x 10(-8) mol.L-1 (P < 0.001); MMP-9 expression induced with phorbol myristate acetate(PMA) was inhibited by dexamethasone at 1 x 10(-9), 1 x 10(-7) and 1 x 10(-5) mol.L-1, indomethacin at 1 x 10(-6) and 1 x 10(-5) mol.L-1 and resveratrol at 1 x 10(-6) and 1 x 10(-5) mol.L-1.
CONCLUSIONThe inhibition of MMP-9 expression may be one of the anti-inflammatory mechanisms of dexamethasone, indomethacin and resveratrol.
