HPLC-ELSD determination of triterpenoids and triterpenoid saponins in Ilex pupurea leaves.
- Author:
Jun-bo XIE
1
;
Zhi-ming BI
;
Ping LI
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; analysis; Glucose; analogs & derivatives; analysis; Ilex; chemistry; Plant Leaves; chemistry; Plants, Medicinal; chemistry; Saponins; analysis; Triterpenes; analysis
- From: Acta Pharmaceutica Sinica 2003;38(7):534-536
- CountryChina
- Language:English
-
Abstract:
AIMTo establish an HPLC-ELSD method for the quantification of pedunculoside, ziyuglycoside I and rutundic acid, in the leaves of Ilex purpurea Hassk.
METHODSBy optimizing the chromatographic conditions of HPLC and the parameters of ELSD to study the methodology. Column: Allsphere ODS-2 (250 mm x 4.6 mm ID, 5 microns), mobile phase: methanol-water (59:41), flow rate: 1.0 mL.min-1, drift tube temperature: 59 degrees C, gas flow rate: 2.38 L.min-1.
RESULTSThe calibration curves were linear in the range of 2.56-25.60 micrograms for pedunculoside, 1.64-16.40 micrograms for ziyuglycoside I and 3.74-37.40 micrograms for rutundic acid. The average recovery of pedunculoside was 96.3%, RSD 1.59% (n = 5), ziyuglycoside I 97.3%, RSD 3.82% (n = 5), rutundic acid 97.7%, RSD 2.11% (n = 5). All of RSDs of the precision were less than 4% (n = 6), and the reproduciblities less than 5% (n = 6).
CONCLUSIONThe method is simple, accurate, effective and feasible. It can be used for the determination of the contents of pedunculoside, ziyuglycoside I and rutundic acid in leaf of Ilex purpurea Hassk.