Determination of first-order structure of somatostatin by electrospray ionization mass spectrometry.
- Author:
Hong-hua ZHOU
1
;
Ren-ling MA
;
Long-sheng SHENG
;
Bing-ren XIANG
;
Deng-kui AN
Author Information
- Publication Type:Journal Article
- MeSH: Amino Acid Sequence; Molecular Structure; Molecular Weight; Somatostatin; analysis; chemistry; Spectrometry, Mass, Electrospray Ionization; methods
- From: Acta Pharmaceutica Sinica 2003;38(8):617-619
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo determine the molecular weight and first-order structure of somatostatin.
METHODSThe molecular weight of somatostatin was determined by electrospray ionization mass spectrometry. Somatostatin was deoxidized by 2-mercaptoethanol. A series of typical fragment ions of deoxidized product were obtained by insource collision-induced dissociation (CID).
RESULTSThe m/z of quasi-molecular ion [M + H]+ of somatostatin was 1,637.8 and [M + Na]+ was 1,659.5. The m/z of double-charge ion [M + 2H]2+ was 819.5 and [M + H + Na]2+ was 830.3. It showed that the molecular weight of somatostatin was 1,636.7. The y and b series of fragment ions of deoxidized product were obtained by adjusting the fragmentor voltage. It was determined that the first-order structure of deoxidized product of somatostatin was A-G-C-K-N-F-F-W-K-T-F-T-S-C.
CONCLUSIONThe molecular weight and first-order structure of somatostatin were confirmed.
