mRNA expression alteration of two-pore potassium channels in the brain of beta-amyloid peptide25-35-induced memory impaired rats.
- Author:
Ya-ping PAN
1
;
Xiang-hua XU
;
Xiao-liang WANG
Author Information
- Publication Type:Journal Article
- MeSH: Amyloid beta-Peptides; Animals; Cerebral Cortex; metabolism; Hippocampus; metabolism; Male; Maze Learning; Memory Disorders; chemically induced; metabolism; Peptide Fragments; Potassium Channels; biosynthesis; genetics; Potassium Channels, Tandem Pore Domain; RNA, Messenger; biosynthesis; genetics; Rats; Rats, Sprague-Dawley; Reverse Transcriptase Polymerase Chain Reaction
- From: Acta Pharmaceutica Sinica 2003;38(10):721-724
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo study mRNA expression alteration of two-pore potassium channels in the brain of beta-amyloid peptide25-35 (beta-AP25-35)-induced memory impaired rats.
METHODSMemory impairments induced in rats by single icv injection of beta-AP25-35 (2 mmol.L-1) 5 microL were assessed in the Morris water maze test. The mRNA expression levels of three two-pore potassium channels TREK-1, TREK-2 and TRAAK were detected in rat cerebral cortex and hippocampus by reverse transcription-polymerase chain reaction (RT-PCR).
RESULTSIn the Morris water maze test, the escape latencies of the beta-AP25-35-treated rats were longer than those of the control group in 1st, 2nd and 4th training day, suggesting that the memory of beta-AP25-35-treated rats was obviously impaired. Compared with the control group, the mRNA levels of TREK-1, TREK-2 and TRAAK in the hippocampus of the beta-AP25-35-treated rats were increased by 40.0%, 27.9% and 18.9%, respectively; while no significant change of TREK-1, TREK-2 and TRAAK mRNA levels was observed in the cortex.
CONCLUSIONThe mRNA expression levels of two-pore potassium channels were increased significantly in the brain of beta-AP25-35-induced memory impaired rats.
