Analysis of the response factors of different quinolones detected by evaporative light-scattering detector.
- Author:
Jian-wen HONG
1
;
Chang-qin HU
;
Long-sheng SHENG
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Ciprofloxacin; analysis; Enoxacin; analysis; Fluoroquinolones; analysis; Levofloxacin; Light; Linear Models; Ofloxacin; analysis; Quinolones; analysis
- From: Acta Pharmaceutica Sinica 2003;38(9):695-697
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo analyze the response factors of different quinolone antibiotics detected by evaporative light-scattering detector (ELSD).
METHODSThe response factors of five different quinolones (enoxacin, levofloxacin, ciprofloxacin, lomefloxacin and gatifloxacin) detected by ELSD were determined by using a YMC-Pack ODS-AM cloumn (150 mm x 4.6 mm ID, 5 microns) as analytical column and 0.5% triethylamine (adjusting pH 2.5 with trifluoroacetic acid)-acetonitrile (48:12) as mobile phase at a flow rate of 0.6 mL.min-1, the temperature of the drift tube was set at 117 degrees C, and the flow of carrier gas at 3.0 L.min-1. Detector responses (A) and the amount of injection of each substance (m) were fitted to the logarithmic regression: log A = b log m + log a.
RESULTSThe linear regression equation obtained were: enoxacin: Y = 1.0799X + 2.7611, r2 = 0.9996; levofloxacin: Y = 1.0913X + 2.7235, r2 = 0.9997; ciprofloxacin: Y = 1.0828X + 2.7523, r2 = 0.9994; lomefloxacin: Y = 1.0891X + 2.7391, r2 = 0.9993; gatifloxacin: Y = 1.0878X + 2.7392, r2 = 0.9995. The differences between them were negligible.
CONCLUSIONDifferent quinolones can give the same responses with ELSD detection. So, the HPLC-ELSD methods can be applied to the determination of new substances by using another substance as reference.
