Stability studies of sulfated polyguluronic acid ester.
- Author:
Zhen-qing ZHANG
1
;
Xia ZHAO
;
Hua-shi GUAN
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, Gel; methods; Drug Stability; Electrophoresis, Polyacrylamide Gel; methods; Molecular Weight; Polysaccharides, Bacterial; chemistry; Spectrophotometry, Ultraviolet; methods; Temperature
- From: Acta Pharmaceutica Sinica 2003;38(9):711-714
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo test the stability of marine polysaccharide drug sulfated polyguluronic acid ester.
METHODSFour methods including high performance gel chromatography (HPGC), poly-acrylamide gel electrophoresis (PAGE), UV scan of absorbance between 200 and 800 nm and gelatin nephelometry were established. Samples were tested in high temperature, high humidity, strong light and accelerated test conditions. The methods were used to test the changes of the parameters including molecular weight, molecular weight distribution, absorbance between 200 and 800 nm, free sulfate, with which we could estimate the stability of sulfated polyguluronic acid ester could be estimated.
RESULTSThe four methods were suitable to test the stability of sulfated polyguluronic acid ester and the sample were stable in the conditions as before except in high temperature.
CONCLUSIONSulfated polyguluronic acid ester has good stability.
