Study on molecular chain morphology and chain parameters of konjac glucomannan.
- Author:
Bin LI
1
;
Bi-jun XIE
Author Information
- Publication Type:Journal Article
- MeSH: Amorphophallus; chemistry; Chromatography, Gel; methods; Light; Mannans; chemistry; isolation & purification; Microscopy, Atomic Force; Molecular Weight; Polysaccharides; chemistry; isolation & purification; Scattering, Radiation; Viscosity
- From: Acta Pharmaceutica Sinica 2003;38(11):838-842
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo study the molecular chain morphology and chain parameters of konjac glucomannan (KGM).
METHODSSolution behavior was studied by using light scattering(LS), gel permeation chromatograph (GPC) and method of viscosity. The molecular morphology was observed directly by atom force microscope (AFM) and transmission electron microscope (TEM).
RESULTSThe average molecular weight (Mw), root-mean-square ratio of gyration(
1/2), second Viral coefficients (A2) and multi-disperse coefficients (Mw/Mn) are 1.04 x 10(6), 105.0 +/- 0.9 nm, (-1.59 +/- 0.28) x 10(-3) mol.mL.g-2 and (1.020 +/- 0.003) respectively; Mark-Houwink equation was established as [eta] = 5.96 x 10(-2) Mw0.73, the molecular chain parameters were as follows: ML = 982.82 nm-1, q = 27.93 nm, d = 0.74 nm, h = 0.26 nm, L = 1,054.11 nm. CONCLUSIONBoth the result showed by direct observation and the deduction drawn by solution behavior confirmed that the KGM molecular is stentering semi-flexible linear chain without branch.
