Secondary structure of insulin encapsulated within liposomes.
- Author:
Xuan ZHANG
1
;
Li-xin HUANG
;
Song-qing NIE
;
Xian-rong QI
;
Qiang ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Drug Carriers; Drug Delivery Systems; Insulin; administration & dosage; chemistry; Liposomes; Protein Structure, Secondary; Spectroscopy, Fourier Transform Infrared
- From: Acta Pharmaceutica Sinica 2003;38(11):863-866
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo determine the secondary structure of insulin encapsulated within liposomes.
METHODSThe secondary structure of insulin, mixture of insulin with liposomes (I) and insulin liposomes (II) were determined by Fourier transform infrared (FTIR) spectroscopy.
RESULTSThe figures of secondary structure of insulin, sample I and II were similar. The results showed that the amount of alpha-helix and beta-sheet of insulin, sample I and II had little difference, from 36.09% (insulin) to 31.68% (sample I) and 31.45% (sample II), and from 47.83% (insulin) to 53.29% (I) and 51.36% (II), respectively. The results showed that the insulin of sample I and II did not insert in liposomes membrane, only adsorbed or extendedon the surface of the liposomes.
CONCLUSIONThe secondary structure of insulin encapsulated within liposomes has not been destroyed and still remain the original state.
