Detection of adenoviruses in feces of infants with diarrhea by Real-time PCR
10.3760/cma.j.issn.0254-6450.2009.04.018
- VernacularTitle:应用实时荧光聚合酶链反应检测婴幼儿腹泻标本中的腺病毒
- Author:
Xiao-Qun ZHENG
1
;
Yan-Yan HUANG
;
Ying PENG
;
Ke HUA
;
Jian-Xin LV
Author Information
1. 温州医学院附属第二医院
- Keywords:
Adenoviruses;
Diarrhea;
Real-time polymerase chain reaction
- From:
Chinese Journal of Epidemiology
2009;30(4):380-383
- CountryChina
- Language:Chinese
-
Abstract:
Objective A Real-time PCR method was established to study the infection of adenovirus (Ad) in infants with sporadic diarrhea in Wenzhou. Methods According to hexon gene of adenovirus, one prime pair was designed as universal primes and applied to detect adenovirus DNA by Real-time PCR. It was also compared with immunochromatographic assay. 157 fecal specimens from diarrhea infants were tested while positive specimens were sequenced and identified by isolate culture and restriction endonucleases. Results A rapid and specific Real-time PCR assay for detection adenovirns was set up. The positive rates of adenovirns in fecal specimens by immunochromatographic assay and Real-time PCR were 1.91% (3/157) and 3.18% (5/157), respectively. Out of the 154 specimens with negative result from immunochromatographic assay, 2 showed positive by Real-time PCR. 5 positive specimens, identified by Real-time PCR, were sequenced as Ad3 (3/157, 1.91% ) and Ad7 (2/157, 1.27% ). 2 of the 5 positive specimens were proved to be Ad3 by cell culture and restriction endonucleases.Conclusion Real-time PCR combined with sequence analysis seemed more sensitive and specific so could be used for identifying types of adenovirus in clinical specimens. Ad3 and Ad7 were important pathogens which caused infant sporadic diarrhea in Wenzhou during February and April in 2008.
