Introduction to the standard operation program of Spoligotyping on Mycobacterium tuberculosis in China
10.3760/cma.j.issn.0254-6450.2009.04.019
- VernacularTitle:中国结核分枝杆菌间隔区寡核苷酸分型方法标准化操作程序的探讨
- Author:
Hai-Yan DONG
1
;
Bing LU
;
Yuan-Yuan ZHANG
;
Zhi-Guang LIU
;
Xiu-Qin ZHAO
;
Yi JIANG
;
Kang-Lin WAN
Author Information
1. 中国疾病预防控制中心传染病预防控制所
- Keywords:
Mycobacterium tuberculosis;
Spacer oligonucleotide typing;
Standard operation program;
Beijing family
- From:
Chinese Journal of Epidemiology
2009;30(4):384-387
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study and preliminarily evaluate the standard spacer oligonucleotide typing (Spoligotyping) method and the application on Mycobacterium tuberculosis (M.tuberculosis).Methods Spoligotyping on 224 M.tuberculosis strains was studied by the molecular biological techniques, including DNA isolation, PCR, reverse line blot hybridization, together with data analysis software BioNumerics (Version 5.0). Results Standardization on both Spoligotyping method and parameters in result analysis and the usage of the analysis software were studied. Through this method, 224 M.tuberculosis clinical strains were classified into 2 clusters including 129 Beijing family strains and 95 non-Beijing family strains. The predominant strains belonged to Beijing family. Conclusion Standard Spoligotyping method was preliminary determined in China, showing that it was a simple, rapid, and robust method for simultaneous detection and typing of M.tubereulosis. This method can be used for tracing the source of infection and understanding the epidemic trend of M.tuberculosis. Spoligotyping can also be served as a method for simultaneous detection and typing ofM.tuberculosis, and to identify Beijing family strains.
