Influence of F protein of hepatitis C virus subtype 1b inhibits on human hepatocellular carcinom HepG2 cell apoptosis

VernacularTitle:丙型肝炎病毒基因1b型F蛋白对人肝细胞癌细胞株HepG2凋亡的影响
Author: Jing-Fing YANG ¹ ; Yun ZHANG ; Xiao-Zhao DENG ; Ke XU ; Zhong-Can WANG ; Jie WANG ; Le FENG ; Wei-Liang DING
Author Information

1. 南京医科大学
Keywords: Hepatitis C virus; F protein; Apoptosis; Tumor necrosis factor α
From: Chinese Journal of Epidemiology 2009;30(4):388-392
CountryChina
Language:Chinese
Abstract: Objective To investigate the effects of F protein of hepatitis C virus subtype lb on the apoptosis of human hepatocellular carcinom HepG2 cells. Methods HepG2 cells were transfected with recombinant plasmid pcDNA3.0-F-EGFP and pcDNA3.0-F-EGFP-HepG2 strain was exposed to Act-D and tumor necrosis factor a (TNFα) treatment in order to induce cell apoptosis with positive control pcDNA3.0-C-EGFP-HepG2, negative control pcDNA3.0-C-EGFP-HepG2 and blank control HepG2.Annexin V-FITC/PI of flow cytometry was performed to determine the number of apoptotic cells. DNA Ladder was used to observe the isolation of apoptotic DNA fragments in the apoptotie cells. Results pcDNA3.0-F-EGFP- HepG2 cell strain showed a much delayed apoptosis as well as obviously lowering the apoptotic rate when compared with the pcDNA3.0-HepG2 strain and HepG2 strain (P<0.001).Conclusion The introduction and expression of extraneous gene (the F gene of hepatitis C virus subtype 1b) could significantly inhibit the apoptosis of HepG2 cells.