Distribution and molecular epidemiologic characterics of insertion sequence IS1301 in Neisseria meningitidis isolated from China
10.3760/cma.j.issn.0254-6450.2009.05.015
- VernacularTitle:中国脑膜炎奈瑟菌中插入序列1301的分布与分子流行病学研究
- Author:
Xing-Lin PANG
1
;
Pei-Bin HOU
;
Yuan GAO
;
Li XU
;
Hong-Yu REN
;
Bing-Qing ZHU
;
Hai-Jian ZHOU
;
Zhu-Jun SHAO
Author Information
1. 广州市疾病预防控制中心
- Keywords:
Neisseria meningitidis;
Insertion sequence 1301;
Pulse field gel electrophoresis;
Southern blot
- From:
Chinese Journal of Epidemiology
2009;30(5):475-480
- CountryChina
- Language:Chinese
-
Abstract:
Objective To research the distribution and molecular epidemiology of insertion sequence IS1301 in Neisseria (N.) meningitidis strains in China, so as to provide scientific and available evidence for a new method of genotyping in N.meningitidis strains with IS1301. Methods Examined the IS1301 by PCR in 219 N.meningitidis strains from 16 provinces and 3 cities during 2007 and 2008 in China, productions of amplification were sent for sequencing. The positive N.meningitidis strains were analyzed by pulse field gel electrophoresis (PFGE) and nucleic acid blotting hybridization(Southern blot) by electrophoresis. Results The positive rates with IS1301 were 15.53%, 11.11%, 20.75%, 6.17% and 28.57% for four serotypes (A, B, C, N) respectively. The sequence comparability between the amplification productions and No.Z49092.1 N.meningitidis which registered in GenBank was 94%-100%. There were two types of clusters devided by cladogram analysis. There appeared large IS1301 sequence difference between the serotype C and others. The number of IS1301 replica ranged from 6-17 per strain at least. The number of IS1301 replica changed in the same type of PFGE N.meningitidis respectively. Conclusion Typing by IS1301 combined with PFGE could comprehend the homology and genetic polymorphism of N.meningitidis epidemic strains at the molecular level.
