Effects of 17β-Estradiol on the Expression of IL-6, IL-11 and NF-κB in Human MG-63 Osteoblast-like Cell Line
- Author:
Yunlin WANG
1
;
Eyuan LIAO
;
Guangda XIANG
;
Ruchun DAI
;
Xinhua XIAO
;
Xianghang LUO
Author Information
1. 华中科技大学同济医学院附属同济医院
- Keywords:
IL-6;
IL-11;
NF-κB;
MG-63
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2006;26(1):53-58
- CountryChina
- Language:Chinese
-
Abstract:
In order to characterize the effects of 17β-estradiol (17β-E2) on the expression of IL-6,IL-11 and NF-κB in the human MG-63 osteoblast-likecell line, the expression of IL-6 was detected by RT-PCR, Northern blot and Western blot. The expression of IL-11 was determined by RT-PCR, and NF-κB by Western blot. The results showed that 17β-E2 down-regulated the expression of IL-6 mRNA and protein, IL-11 mRNA and NF-κB protein in MG-63 cells. It was suggested that the expression of NF-κB, IL-6 and IL-11 in MG-63 cells could be suppressed by 17β-E2, and this might lend support to estrogen replacement therapy in postmenopausal women.
