Detection of Human Parvovirus B19 Nonstrutural Protein DNA by Nested-Polymerase Chain Reaction in Gravida Serum and Pregnant Tissues
- Author:
Ting SHEN
1
;
Yongmei HUANG
;
Fuyuan QIAO
;
Zengqing LI
;
Haiyi LIU
Author Information
1. 华中科技大学同济医学院
- Keywords:
parvovirus B19,human;
nested-polymerase chain reaction;
nonstrutural protein;
pregnancy
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2006;26(1):123-126
- CountryChina
- Language:Chinese
-
Abstract:
A new nested-polymerase chain reaction (nested-PCR) assay was developed to detect human parvovirus B19 DNA corresponding to the nonstructural protein in clinical specimens in a routine diagnostic laboratory. The sensitivity of this highly specific assay was up to 0. 005 fg of B19 DNA. Parvovirus B19 was identified in sera of 20 pregnant women with abnormal pregnant outcome. Among these 20 cases, intrauterine parvovirus infection did exist in 7 pregnant women because parvovirus B19 DNA was detected in the pregnant tissues of them such as placenta tissues,chorionic villi, amniotic fluid, fetal spleen, liver and abdominal fluids.
