Effect of Ca2+i and Neuronal Mitochondria Transmembrane Potentials in Hippocampus of Murine Cytomegalovirus Infected Mice
- Author:
Ying ZHANG
1
;
Liangzhen WEN
;
Biheng CHENG
Author Information
1. 华中科技大学同济医学院附属同济医院
- Keywords:
cytomegalovirus;
infection;
hippocampus;
calcium;
mitochondria transmembrane potential
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2006;26(2):211-212
- CountryChina
- Language:Chinese
-
Abstract:
To explore the effect of [Ca2+]I and neuronal mitochondria transmembrane potentials in hippocampus of murine cytomegalovirus (MCMV) infected mice, newborn Balb/c mice were randomly divided into two groups: a virus inoculated group and a control group. After 56 days, single cell of hippocampus was isolated, and mitochondria transmembrane potentials and the intracellular free calcium level [Ca2+ ]I in hippocampus were measured by means of flow cytometry (FCM).Compared with the control group, the mitochondria transmembrane potentials was decreased (P＜0.01) and the intracellular free calcium level [Ca2+]I was increased (P＜0.01) in inoculated group.The dysfunction of [Ca2+ ]I and mitochondria transmembrane potentials in hippocampus may play an important role in the functional disorders in CMV-infected CNS.
