Relationship between Single Nucleotide Polymorphism in TNF-α Gene Promoter Region and Inhibitory Effects of Triptolide on TNF-α Production in Peripheral Blood Mononuclear Cells of Healthy Humans
- Author:
Shenghao TU
1
;
Hongbo CHEN
;
Dongyun SHENG
;
Yonghong HU
;
Peilin LIU
Author Information
1. 华中科技大学同济医学院附属同济医院
- Keywords:
tumor necrosis factor-α;
gene polymorphism;
triptolide;
peripheral blood mononuclear cells
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2006;26(3):347-349
- CountryChina
- Language:Chinese
-
Abstract:
The relationship between tumour necrosis factor-α (TNF-α) gene polymorphism and inhibitory effects of triptolide on TNF-α production from peripheral blood mononuclear cells (PBMC)of healthy humans was investigated. Genomic DNA from 41 healthy people was typed for TNF-α-308 polymorphism by allele-specific polymorphism chain reaction (AS-PCR). The TNF-α concentration in the supernatant was measured by ELISA. The results showed that the production of TNF-α from TNF-α -308 non-G/G genotype PBMC was higher than that from TNF-α-308 G/G genotype PBMC after stimulated by LPS. Triptolide could lower the production of TNF-α from G/G genotype PBMC, but had no effect on the level of TNF-α from non-G/G genotype PBMC. It was concluded that TNF-α gene polymorphism was related to the TNF-α production from triptolide-inhibited PBMC culture in healthy humans.
