Expression of MDR1 and KIT in imatinib-resistant gastrointestinal stromal tumor cells.
- Author:
Jing-lei LIU
1
;
Jing QIN
;
Li-qing YAO
;
Ying-yong HOU
;
Yi-hong SUN
;
Kun-tang SHEN
;
Ping SU
Author Information
- Publication Type:Journal Article
- MeSH: ATP-Binding Cassette, Sub-Family B, Member 1; genetics; Benzamides; Cell Adhesion Molecules; genetics; Cell Line, Tumor; Drug Resistance, Neoplasm; genetics; Gastrointestinal Stromal Tumors; genetics; Humans; Imatinib Mesylate; Piperazines; pharmacology; Protein Kinase Inhibitors; pharmacology; Proto-Oncogene Proteins c-kit; genetics; Pyrimidines; pharmacology
- From: Chinese Journal of Gastrointestinal Surgery 2010;13(7):506-509
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the relationship between imatinib resistance and genes MDR1 and KIT in gastrointestinal stromal tumor (GIST) cells.
METHODSThe MDR1 and KIT mRNA level in GIST882-R and GIST882-S cells were detected by RT-PCR. Immunocytochemistry and Western blot were employed to detect P-gp and CD117 expression in GIST882-R and GIST882-S cells.
RESULTSThe relative expression of MDR1 mRNA was 0.321 + or - 0.033 in GIST882-R and 0.157 + or - 0.056 in GIST882-S cells, and the difference was statistically significant (P<0.05). The relative expression of KIT mRNA was 0.389 + or - 0.063 in GIST882-R and 0.339 + or - 0.067 in GIST882-S, and the difference was not statistically significant (P>0.05). The relative density of P-gp was 0.443 + or - 0.058 in GIST882-R and 0.237 + or - 0.094 in GIST882-S, and the difference was statistically significant (P<0.05). The relative density of CD117 was 0.744 + or - 0.123 in GIST882-R and 0.704 + or - 0.094 in GIST882-S, and the difference was not statistically significant (P>0.05).
CONCLUSIONSOver-expression of gene MDR1 may be associated with imatinib resistance in GIST. KIT may not be involved in imatinib resistance.
