Significance of IL-1beta-induced ectopic expression of CDX2 in the intestinal metaplasia of gastric epithelium.
- Author:
Jiang LI
1
;
Guo-bin WANG
;
Ren-hu SUN
;
Kai-xiong TAO
Author Information
- Publication Type:Journal Article
- MeSH: CDX2 Transcription Factor; Cell Line; Epithelium; metabolism; pathology; Gastric Mucosa; cytology; metabolism; pathology; Homeodomain Proteins; metabolism; Humans; Interleukin-1beta; pharmacology; Metaplasia; RNA, Messenger; genetics
- From: Chinese Journal of Gastrointestinal Surgery 2010;13(7):524-527
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the effect of IL-1beta on the expression of CDX2 in human gastric epithelial cell line GES-1 and its role in the intestinal metaplasia.
METHODSGES-1 cells were treated with IL-1beta in different concentrations and the expressions of CDX2 mRNA and protein were detected by real-time PCR, immunocytochemistry and Western blot at different time points. GES-1 cells were then pre-treated with NF-KappaB pathway inhibitor PDTC, and the expression of CDX2 mRNA and protein induced by IL-1beta were detected. The cell ultra-structure of GES-1 cells was observed by electronic microscope after GES-1 being treated with IL-1beta for 25 days.
RESULTSLevels of CDX2 mRNA and protein were 0.0749 + or - 0.0021 and 0.56 + or - 0.04 in the cells treated with 1 microg/L IL-1beta(P<0.05). After pre-treatment with PDTC, levels of CDX2 mRNA and protein were 0.0006 + or - 0.0002 and 0.40 + or - 0.06(P<0.05). Some changes in the cell ultra-structure of GES-1 were found by electronic microscope when GES-1 was treated with IL-1beta for 25 days.
CONCLUSIONIL-1beta can stimulate CDX2 mRNA and protein expression in GES-1 cells through the NF-KappaB signal pathway, indicating that IL-1beta plays an important role in the intestinal metaplasia.
