Expression of Na+-H+ exchanger 1 in human gastric carcinoma tissue and its clinical significance.
- Author:
Yan-yang NIU
1
;
Pei-wu YU
;
Bo TANG
;
Yan SHI
;
Ying-xue HAO
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Carcinoma; metabolism; pathology; Cation Transport Proteins; metabolism; Female; Humans; Male; Middle Aged; Prognosis; RNA, Messenger; metabolism; Sodium-Hydrogen Exchanger 1; Sodium-Hydrogen Exchangers; metabolism; Stomach; metabolism; pathology; Stomach Neoplasms; metabolism; pathology
- From: Chinese Journal of Gastrointestinal Surgery 2010;13(8):604-607
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine the expression of Na+/H+ exchanger 1(NHE1) in human gastric carcinoma tissue and to investigate the association between NHE1 expression and clinicopathological characteristics.
METHODSThe expressions of NHE1 mRNA and protein were detected in both gastric carcinoma tissue (n=60) and adjacent gastric mucosa tissue (n=30) by reverse transcription polymerase chain reaction (RT-PCR) and Western blot. The association between the expression and the clinicopathological characteristics was analyzed.
RESULTSThe relative expression levels of NHE1 mRNA and protein in gastric carcinoma tissue were 0.786+/-0.291 and 1.442+/-0.175, which were significantly higher than those in adjacent gastric mucosa tissue (0.369+/-0.052 and 0.348+/-0.029) (P<0.01). The expression of NHE1 mRNA was positively correlated with NHE1 protein in the gastric carcinoma tissue (r=0.264, P<0.05). The expressions of NHE1 mRNA and protein were associated with the depth of invasion, lymph node metastasis, and TNM staging (P<0.05). However, no statistical difference was found in age, gender, and tumor differentiation (P>0.05).
CONCLUSIONThe expression levels of NHE1 mRNA and protein are significantly up-regulated in gastric carcinoma tissue, which may be involved in the development of gastric carcinoma.
