Effect of COX-2 inhibitor on the expression of BCL-3 and cyclin D1 in human colon cancer cell line SW480.
- Author:
Shao-min WANG
1
;
Meng YE
;
Shu-min NI
;
Xiao WU
;
Guang YANG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line, Tumor; drug effects; Colonic Neoplasms; metabolism; Cyclin D1; metabolism; Cyclooxygenase 2 Inhibitors; pharmacology; Humans; Nitrobenzenes; pharmacology; Proto-Oncogene Proteins; metabolism; Sulfonamides; pharmacology; Transcription Factors; metabolism
- From: Chinese Journal of Gastrointestinal Surgery 2010;13(8):612-615
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects of NS398, a selective cyclooxygenase-2 (COX-2) inhibitor, on the transcription and translation of BCL-3 and its regulatory gene cyclin D1 in colon cancer cell line SW480.
METHODSHuman colon cancer cells SW480 were divided into two groups: SW480 cells in experimental group were treated with NS398 in different concentrations(25 micromol/L, 50 micromol/L, 100 micromol/L and 200 micromol/L) for 48 h or 72 h. SW480 cells in control group were treated with media which did not contain NS398. Then the expressions of BCL-3 and cyclin D1 were detected by RT-PCR, Western blot, and immunocytochemistry.
RESULTSAt 48 hours RT-PCR showed that BCL-3 mRNA and cyclin D1 mRNA decreased in a dose-dependent manner in the experimental group. However, there were no significant differences in the levels of BCL-3 protein and cyclin D1 protein between two groups (P>0.05). At 72 hours, BCL-3 protein and cyclin D1 protein also decreased in a dose-dependent manner in the experimental group. When the concentration of NS398 reached 100 micromol/L, the differences between the two groups in the expression of BCL-3 mRNA and protein became statistically significant (P<0.01). When the concentration of NS398 reached 50 micromol/L, the differences in the expression of cyclin D1 mRNA and protein were statistically significant (P<0.05).
CONCLUSIONSBCL-3 is expressed in colon cancer cell line SW480. COX-2 inhibitor can inhibit the expression of BCL-3 and cyclin D1 in a dose-dependent manner. NS398 may down-regulate the expression of cyclin D1 through BCL-3.