Determination of GATA-4 in the testis of the mouse.
- Author:
Jia-Zhou YANG
1
;
Yu-Feng ZHAO
;
Yan-Mei WANG
;
Cai-Xia JING
;
Nai-Zhou MIAO
;
Qing-Yan AI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Differentiation; GATA4 Transcription Factor; metabolism; Germ Cells; metabolism; Leydig Cells; metabolism; Male; Mice; Sertoli Cells; metabolism; Testis; cytology; metabolism
- From: National Journal of Andrology 2010;16(10):901-904
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the characteristics and distribution of GATA-4 in the testis of male mice.
METHODSParaffin sections were obtained from the testes of 24 male B6SJLF1/J mice, aged 0 day (n = 6), 2 weeks (n = 6), 4 weeks (n = 6) and 6 weeks (n = 6), and the expressions of GATA-4 in the testis were observed by the immunohistochemical ABC method and DAB visualization at different times.
RESULTSPositive expressions of GATA4 were found in the Sertoli cells and Leydig cells of all the mice, but significantly higher in the 4- and 6-week-old than in the 0-day and 2-week-old groups (P < 0.01). And they were also observed in the germ cells of the 4- and 6-week-old mice, significantly higher in the latter than in the former (P < 0.01).
CONCLUSIONGATA-4 exists in the testis of male mice, which has provided a morphological base for sex determination and differentiation and hormone regulation in the testis.
