Expression of CR16 in the testis of patients with idiopathic azoospermia.
- Author:
Zi-Na WEN
1
;
Wen-Juan PANG
;
Wen-Pei XIANG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Azoospermia; metabolism; pathology; Case-Control Studies; Cytoskeletal Proteins; metabolism; Humans; Male; Testis; metabolism; pathology
- From: National Journal of Andrology 2010;16(12):1101-1104
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression of corticosteroids and regional expression 16 (CR16) in the testis of patients with idiopathic azoospermia and the role of CR16 in spermatogenesis.
METHODSImmunohistochemistry and RT-PCR were used to detect the expression levels of the CR16 protein and mRNA in the testes of 48 patients with idiopathic azoospermia and 10 healthy men.
RESULTSImmunohistochemistry showed that the CR16 protein expressed in the Sertoli cells and spermatids-binding region in the epithelium of seminiferous tubules. The level of the CR16 protein was markedly lower in the idiopathic azoospermia patients than in the healthy men, and RT-PCR also showed a significantly decreased level of CR16 mRNA in the testis of the patients.
CONCLUSIONThe expressions of the CR16 protein and mRNA decrease markedly in the testis of patients with idiopathic azoospermia, indicating a correlation with the pathogenesis of azoospermia.
