Hypoxia induces fibrosis of corpus cavernosum smooth muscle in SD rats.
- Author:
Bo-dong LÜ
1
;
Jian-hua NIAN
;
Xiao-jun HUANG
;
Shi-geng ZHANG
;
Qiang GENG
;
Gang CHEN
;
Shi-tao CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Hypoxia; Cells, Cultured; Collagen Type I; metabolism; Extracellular Matrix; Fibrosis; Male; Muscle, Smooth; cytology; pathology; Oxygen; metabolism; Penis; metabolism; pathology; Rats; Rats, Sprague-Dawley; Transforming Growth Factor beta1; metabolism
- From: National Journal of Andrology 2011;17(2):121-125
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effect of hypoxia on the fibrosis of corpus cavernosum smooth muscle cells (CCSMC) in SD rats.
METHODSCCSMCs were cultured in vitro, identified by immunohistochemistry, and then exposed to hypoxia at the concentration of 1% O2 for 12, 24, 48 and 72 hours. Those exposed to normal oxygen concentration for the corresponding lengths of time were used as the control. The relative expressions of TGF-131, type I collagen and type DI collagen were determined by RT-PCR.
RESULTSThe in vitro cultured CCSMCs grew well, and the anti-a-smooth muscle actin monoclonal antibodies were positive on immunohistochemical staining. The relative expression levels of TGF-beta1, type I collagen and type mI collagen were positively correlated with the time of hypoxia interference within 48 hours, and did not increase further with prolonged exposure.
CONCLUSIONWhen exposed to hypoxia, the relative expressions of TGF-beta1, type I collagen and type mI collagen in the CCSMCs of SD rats increased with the length of time, and reached the peak at 48 hours. Hypoxia can cause fibrosis of CCSMCs in SD rats.
