Inhibitory effect of solanine on prostate cancer cell line PC-3 in vitro.
- Author:
Jun ZHANG
1
;
Guo-wei SHI
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; drug effects; Cell Cycle; drug effects; Cell Line, Tumor; Cell Proliferation; drug effects; Humans; I-kappa B Proteins; metabolism; Male; NF-KappaB Inhibitor alpha; Prostatic Neoplasms; metabolism; pathology; Proto-Oncogene Proteins c-bcl-2; metabolism; Solanine; pharmacology
- From: National Journal of Andrology 2011;17(3):284-287
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the mechanisms of the effects of solanine on human androgen-independent prostate cancer cell line PC-3 in vitro.
METHODSPC-3 cells were treated with solanine at the concentration of 0, 30, 40 and 50 microg/ml, and the cell activity was measured by CCK-8 at 12, 24 and 48 hours after the treatment. At 24 hours, the cell cycle and apoptosis were detected by flow cytometry and fluorescence microscopy, and the protein expressions of I(kappa)B(alpha) and Bcl-2 determined by Western blot.
RESULTSSolanine suppressed the growth of PC-3 cells in a dose- and time-dependent manner in vitro, with significant differences among different concentration and time groups (P < 0.05). The cycle of the PC-3 cells was arrested in the S phase (P < 0.05), with a significantly higher rate of apoptosis in the treated groups than in the controls (P < 0.05). The protein expression of I(kappa)B(alpha) was obviously up-regulated and that of Bcl-2 down-regulated in all the solanine concentration groups.
CONCLUSIONSolanine has an anti-prostate cancer effect by inhibiting PC-3 cell proliferation, arresting the S phase, inducing cell apoptosis, up-regulating the protein expression of I(kappa)B(alpha) and down-regulating that of Bcl-2.
