Effects of a microfluidic sperm sorter on sperm routine parameters and DNA integrity.
- Author:
Wei WANG
1
;
Guang-Tie LIANG
;
Ya-Ya PENG
;
Da-Yu LIU
;
Xiao-Mian ZHOU
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Cell Separation; instrumentation; methods; DNA; DNA Damage; Humans; Infertility, Male; genetics; physiopathology; Male; Microfluidics; Middle Aged; Protein Array Analysis; Semen Analysis; instrumentation; methods; Sperm Motility; Spermatozoa
- From: National Journal of Andrology 2011;17(4):301-304
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of a microfluidic sperm sorter on the routine parameters and DNA integrity of human sperm.
METHODSWe divided 40 semen samples into two aliquots and performed sperm sorting using a self-made polydimethylsiloxane microfluidic sperm sorter and the swim-up method, respectively. Then we evaluated and compared the effects of these two methods on the sperm routine parameters and DNA integrity by computer-assisted sperm analysis and sperm chromatin dispersion test.
RESULTSAfter processing, sperm motility, normal morphology and tail hypoosmotic swelling rate were significantly improved, while sperm DNA damage remarkably decreased (P < 0.01). The microfluidic sperm sorter achieved a significantly lower rate of sperm DNA damage than the swim-up method ([ 8.4 +/- 5.8 ]% vs [16.4 +/- 9.2] %, P < 0.01), but no statistically significant differences were found in all other parameters between the two methods.
CONCLUSIONHigh-quality sperm with less DNA integrity damage could be obtained in sperm sorting with the microfluidic sperm sorter.
