Characterization of prostate cancer cell lines and their epithelial-mesenchymal transition in subcutaneous tumors.
- Author:
Lin ZHAO
1
;
Yong-Guang JIANG
;
Jie MA
;
Yong LUO
;
Jia-Hui ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cadherins; metabolism; Cell Dedifferentiation; Cell Line, Tumor; Cell Transformation, Neoplastic; metabolism; pathology; Epithelial-Mesenchymal Transition; Humans; Male; Mice; Mice, SCID; Prostatic Neoplasms; metabolism; pathology; Subcutaneous Tissue; pathology; Vimentin; metabolism
- From: National Journal of Andrology 2011;17(4):314-317
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo identify the differences in the expression of epithelial or mesenchymal standard proteins between prostate cancer cell lines and tumors, and to investigate the relationship between the process of the prostate cancer cell line forming subcutaneous tumors and epithelial-mesenchymal transition (EMT) by comparing the characteristics of different prostate cell lines forming subcutaneous tumors in SCID mice.
METHODSWe constructed prostate cancer models in male SCID mice by subcutaneous injection of 4 human prostate cancer cell lines DU145, Tsu, PC3 and LNCaP, and compared the characteristics of tumor formation. We used Western blot to detect the expressions of E-cadherin and Vimentin in the cancer cell lines and subcutaneous tumors, observed their differences before and after tumor formation, and explore the relationship between EMT and tumor formation.
RESULTSThe EMT positive cells DU145 and Tsu showed a higher rate and speed of tumor formation than the EMT negative ones PC3 and LNCaP. The expression of E-cadherin was down-regulated in DU145, up-regulated in Tsu, and absent in PC3 and LNCaP.
CONCLUSIONEMT positive cells have a stronger ability of forming tumors than EMT negative cells, and mesenchymal-epithelial transition does exist in subcutaneous tumor formation.
