Effects of nonylphenol and cadmium on sperm acrosome reaction in vitro in mice.
- Author:
Zhu-Xiong SHAO
1
;
Hong-Tao JIANG
;
Fang LIANG
;
Bao-Chang ZHU
Author Information
- Publication Type:Journal Article
- MeSH: Acrosome; drug effects; Acrosome Reaction; drug effects; Animals; Cadmium; pharmacology; In Vitro Techniques; Male; Mice; Mice, Inbred C57BL; Phenols; pharmacology; Spermatozoa; drug effects
- From: National Journal of Andrology 2011;17(4):318-321
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects of nonylphenol and cadmium on acrosome reaction in vitro in mouse spermatozoa.
METHODSSperm were collected from the vas deferens of mice, capacitated in vitro and stimulated with A23187 at 30 micromol/L to induce acrosome reaction. Then the sperm suspension was treated with nonylphenol at 10, 20, 30, 60 and 100 micromol/L or cadmium at 500, 2500 and 5 000 micromol/L, and the control group treated with the carrier solvent. Acrosome reaction of the sperm was analyzed by FITC-PSA staining.
RESULTSCompared with the control group, nonylphenol significantly inhibited acrosome reaction at the concentration of > 60 micromol/L (P < 0.01), but not at < 30 micromol/L (P > 0.05), and the sperm survival rate was reduced with increased concentration of nonylphenol. However, cadmium exhibited no significant influence on either acrosome reaction (P > 0.05) or sperm survival rate at 500 - 5 000 micromol/L.
CONCLUSIONNonylphenol and cadmium affect the spermatogenesis of mice in different ways; the former directly inhibits sperm acrosome reaction, while the latter has no direct effect on it.
