Assessment of the application of variable-number tandem repeat loci of Salmonella Enteritidis in subtyping multiple-locus variable-number tandem repeat analysis.
- Author:
Li ZHANG
1
;
Hui HAN
;
Hai-jian ZHOU
;
Zhi-gang CUI
;
Mei-ying YAN
;
Li-juan ZHANG
;
Biao KAN
Author Information
- Publication Type:Journal Article
- MeSH: Bacterial Typing Techniques; methods; Electrophoresis, Gel, Pulsed-Field; Minisatellite Repeats; Multilocus Sequence Typing; methods; Salmonella enteritidis; classification; genetics
- From: Chinese Journal of Preventive Medicine 2011;45(6):516-521
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the feasibility of the application of variable-number tandem repeat (VNTR) loci of Salmonella Enteritidis (S. enteritidis) in subtyping mutiple-locus variable-number tandem repeat analysis (MLVA).
METHODSA total of 16 isolates of S.enteritidis from different place and time in China were preliminarily assessed by choosing 11 reported VNTR loci, the loci with single amplified bands were picked to subtype all 104 S. enteritidis isolates. The isolates were also analyzed by pulse field gel electrophoresis (PFGE) to compare the superiority or inferiority of MLVA method and PFGE method.
RESULTSSeven VNTR loci were selected from the preliminary screening to expand the analysis, and the 7 VNTR loci had grouped 104 of S.enteritidis isolates into either 16 MLVA subtypes or 22 PFGE subtypes, with the D value at 0.7222 and 0.7974, respectively. Comparing with the isolates in MLVA subtypes, the isolates in PFGE showed a stronger resolving power. Meanwhile the results in PFGE showed a more disperse frequency distribution than those in MLVA.
CONCLUSIONThese results indicate that some VNTR locus which have shown a good polymorphism internationally, may fail to show polymorphism in China, thereby, more VNTR loci should be included in MLVA and the wide screening may benefit the unity of global laboratorial methods.
