Comparative proteomic analysis of rat juvenile and adult dura.
- Author:
Tao SONG
1
;
Nan BI
;
Zhe PENG
;
Lai GUI
;
Bao-sheng CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Aging; physiology; Animals; Blotting, Western; Dura Mater; chemistry; Electrophoresis; Mass Spectrometry; Proteins; analysis; Proteomics; Rats; Rats, Sprague-Dawley
- From: Chinese Medical Journal 2008;121(18):1815-1820
- CountryChina
- Language:English
-
Abstract:
BACKGROUNDIt has been widely observed that infants and young children can reossify large calvarial defects when they are younger than 2 years of age; afterwards, they lose this regenerative potential. Previous studies have implicated that the dura mater serves as a key regulator of calvarial regeneration. However, the molecular mechanism of calvarial reossification remains elusive.
METHODSIn order to identify the proteins that may participate in this process, we performed a proteome-wide comparison of the protein expression levels of immature and mature dura using 2D electrophoresis and MALDI-TOF mass spectrometry. The Western blotting was used to verify the results of the 2D electrophoresis/MALDI-TOF mass spectrometry.
RESULTSEleven proteins were found to express with significant differences in the immature and the mature dura. Among them, the emergence of vimentin, tropomyosin, beta-actin and gamma-actin were further confirmed by the Western blotting analysis.
CONCLUSIONThe proteins and proteomic profiles provide a better understanding of the molecular mechanism of calvarial regeneration.