Inducible nitric oxide synthase is involved in the oxidation stress induced by HIV-1 gp120 in human retina pigment epithelial cells.
- Author:
Qiu-rong YU
1
;
Zhen-ping ZHANG
;
Hui ZHANG
;
Hao-tian LIN
;
Xiu-mei LI
;
Ling BAI
;
Wei-bin CAI
Author Information
- Publication Type:Journal Article
- MeSH: Blotting, Western; Cell Line; Epithelial Cells; drug effects; metabolism; HIV Envelope Protein gp120; pharmacology; Humans; Immunohistochemistry; Microscopy, Confocal; Nitric Oxide; metabolism; Nitric Oxide Synthase Type II; genetics; metabolism; Oxidative Stress; drug effects; Polymerase Chain Reaction; Retina; cytology
- From: Chinese Medical Journal 2008;121(24):2578-2583
- CountryChina
- Language:English
-
Abstract:
BACKGROUNDThe human immunodeficiency virus-1 (HIV-1) envelope glycoprotein gp120 has been implicated in the development of AIDS-associated retinopathy. The present study tested the hypothesis that gp120 may induce oxidative stress including up regulation of inducible nitric oxide synthase (iNOS) and production of malondialdehyde (MDA) and nitric oxide (NO) to mediate retinopathy in retinal pigment epithelial (RPE) cells.
METHODSHuman RPE cell line D407 was cultured and treated with gp120. HIV-1 gp120 protein induced lipid peroxidation product MDA. NO production and iNOS expression were examined in vitro by spectrophomtometry, real-time PCR, Western blotting, and confocal microscope.
RESULTSAddition of gp120 was able to induce RPE cells to produce NO and MDA in time- and dose-dependent manners (P < 0.05). Similarly, gp120 was also capable of up-regulating iNOS mRNA and protein in D407 cells in time- and dose-dependent manners.
CONCLUSIONSGp120 induces oxidative stress in D407 cell by stimulating MDA and NO production, which is mediated by up-regulating iNOS expression. Gp120 may mediate oxidation stress in AIDS-associated retinopathy.
