Gastric Autoantigenic Proteins in Helicobacter Pylori Infection.
10.3349/ymj.2013.54.6.1342
- Author:
Ji Sook PARK
1
;
Su Jin LEE
;
Tae Hyo KIM
;
Jeongsuk YEOM
;
Eun Sil PARK
;
Ji Hyun SEO
;
Jin Su JUN
;
Jae Young LIM
;
Chan Hoo PARK
;
Hyang Ok WOO
;
Hee Shang YOUN
;
Gyung Hyuck KO
;
Hyung Lyun KANG
;
Seung Chul BAIK
;
Woo Kon LEE
;
Myung Je CHO
;
Kwang Ho RHEE
Author Information
1. Department of Pediatrics, Gyeongsang National University School of Medicine, Gyeongsang Institute of Health Science, Jinju, Korea. hsyoun@gnu.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Helicobacter pylori;
gastric atrophy;
autoantigen;
2D immunoblotting
- MeSH:
Alcohol Dehydrogenase/metabolism;
Autoantigens/*metabolism;
Electrophoresis, Gel, Two-Dimensional;
Gastric Mucosa/metabolism/microbiology;
Helicobacter Infections/*metabolism;
Humans;
Peptide Hormones/metabolism;
Phosphopyruvate Hydratase/metabolism
- From:Yonsei Medical Journal
2013;54(6):1342-1352
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: This study tried to identify novel gastric autoimmune antigens that might be involved in aggravating the atrophic gastritis among patients with Helicobacter pylori infection using two-dimensional immunoblotting analysis. MATERIALS AND METHODS: Proteins from gastric mucosal antrectomy specimens and AGS cells (gastric adenocarcinoma cell lines derived from a Caucasian patient who had received no prior therapy) were 2-dimensionally immunoblotted separately with a pool of 300 sera from H. pylroi-infected patients at Gyeongsang National University Hospital. RESULTS: Thirty-eight autoantigenic proteins including alcohol dehydrogenase [NADP+], alpha enolase, gastrokine-1, gastric triacylglycerol lipase, heat shock 70 kDa protein 1, and peroxiredoxin-2 were identified in the gastric mucosal tissue. Fourteen autoantigenic proteins including programmed cell death 6-interacting protein, serum albumin and T-complex protein 1 subunit gamma were identified in the AGS cells. Albumin, alpha-enolase, annexin A3, cytoplasmic actin 1, heat shock cognate 71 kDa protein and leukocyte elastase inhibitor were commonly observed autoantigenic proteins in both gastric mucosal tissue and AGS cells. Alpha-enolase, glutathione S-transferase P, heat shock cognate 71 kDa protein, heat shock 70 kDa protein 1, human mitochondrial adenosine triphosphate synthase (ATP) subunit beta, mitochondrial 60 kDa heat shock protein, peroxiredoxin-2, 78 kDa glucose-regulated protein precursor, tyrosine-protein phosphatase non-receptor type 11 and Tryptophan-Aspartic acid (WD) repeat-containing protein 1 showed 60% or higher amino acid positivity. CONCLUSION: These newly identified gastric autoimmune antigens might be useful in the control and prevention of gastroduodenal disorders, and might be valuable in breaking the vicious circle that exists in gastroduodenal disorders if their pathophysiological roles could be understood in the progress of chronic atrophic gastritis, gastroduodenal ulcers, intestinal metaplasia, and gastric carcinogenesis.
