Effects of CD40LIg gene-modified bone marrow mesenchymal stem cells on liver graft rejection in rats.
- Author:
Jian ZHANG
1
;
Qi ZHANG
;
Guo-ying WANG
;
Ying-cai ZHANG
;
Huan-bing ZHU
;
Yang YANG
Author Information
- Publication Type:Journal Article
- MeSH: Adenoviridae; genetics; metabolism; Animals; Bone Marrow Cells; cytology; metabolism; Graft Rejection; prevention & control; Liver Transplantation; adverse effects; Male; Mesenchymal Stem Cell Transplantation; methods; Mesenchymal Stromal Cells; cytology; metabolism; Rats; Rats, Inbred Dahl; Rats, Inbred Lew; Recombinant Fusion Proteins; biosynthesis; genetics; Recombinant Proteins; biosynthesis; genetics; Transfection
- From: Journal of Southern Medical University 2011;31(11):1903-1906
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects of cluster of differentiation 40 ligand immunoglobulin (CD40LIg) gene-modified bone marrow mesenchymal stem cells (MSCs) on liver graft rejection in rats.
METHODSThe orthotopic liver transplantation models were established with DA rats as the donors and Lewis rats as the recipient. MSCs infected with the recombinant adenoviruses containing CD40LIg gene were infused into the liver graft after transplantation. The liver function, survival of the recipient rats and the morphological changes of the liver grafts were observed after the transplantation. The serum levels of the cytokines interferon-γ (INF-γ) and interleukin-2 (IL-2) in the recipient rats were quantified by ELISA.
RESULTSThe survival of the recipient rats receiving transplantation of genetically modified MSCs (group D) was significantly prolonged compared with that of the control group (group A), MSCs group (group B) and gene transfection group (group C); the survival of groups B and C were significantly longer than that of group A (F=7.615, P<0.05). The level of serum alanine aminotransferase, total bilirubin, IL-2 and INF-γ were significantly higher in group A than in the other 3 groups (F=8.738, P<0.05). HE staining of the liver grafts showed severe acute rejection in group A, mild acute graft rejection in groups B and group C, but no rejection in group D.
CONCLUSIONCD40LIg gene-modified MSCs can prolong the survival of the recipient rats and suppress graft rejection following liver transplantation.
