Expression of MMP-2 and TIMP-1 in renal tissues of patients with chronic active antibody-mediated renal graft rejection.
- Author:
Bao-yao WANG
1
;
Qiang YAN
;
He-qun ZOU
;
Wei-guo SUI
;
Gui-mian ZUO
;
Gui-rong LIANG
;
Hao LUO
;
Shui-yong XIE
;
Huai-zhou CHEN
;
Shen-ping XIE
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Antibody Formation; Complement C4b; metabolism; Female; Fibrosis; etiology; Graft Rejection; immunology; Humans; Kidney; metabolism; Kidney Diseases; pathology; Kidney Transplantation; adverse effects; immunology; Male; Matrix Metalloproteinase 2; genetics; metabolism; Middle Aged; Peptide Fragments; metabolism; Tissue Inhibitor of Metalloproteinase-1; genetics; metabolism
- From: Journal of Southern Medical University 2011;31(12):2048-2051
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expressions of matrix metalloprotein-2 (MMP-2) and tissue inhibitor of metallopeptidase inhibitor-1 (TIMP-1) in the renal allografts of patients with chronic active antibody-mediated rejection (ABMR), and explore their role in the pathogenesis of ABMR.
METHODSImmunohistochemistry and computer-assisted image analysis were used to detect the expression of MMP-2 and TIMP-1 in the renal allografts of 46 patients with interstitial fibrosis and tubular atrophy (IF/TA), with 15 normal renal tissue specimens as the control. The association of MMP-2 and TIMP-1 with the pathological grade of IF/TA in ABMR was analyzed.
RESULTSThe expressions of MMP-2 and TIMP-1 significantly increased in the renal tissues of the patients as compared with the normal renal tissues (P<0.05). MMP-2 expression tended to decrease, while TIMP-1 and serum creatinine increased with the pathological grades of IF/TA (P<0.05). In IF/TA group, the expression of TIMP-1 was positively correlated to serum creatinine level (r=0.718, P=0.00<0.05).
CONCLUSIONAbnormal expressions of MMP-2 and TIMP-1 can promote the development of renal fibrosis in chronic ABMR.