Effect of 25-hydroxyvitamin D(3) on vitamin D receptor expression and distribution in human bronchial epithelial cells in vitro.
- Author:
Hangming DONG
1
;
Haijin ZHAO
;
Laiyu LIU
;
Zhenyu LIANG
;
Yanhua LV
;
Shaoxi CAI
Author Information
- Publication Type:Journal Article
- MeSH: Bronchi; cytology; Calcifediol; pharmacology; Cell Line; Epithelial Cells; cytology; metabolism; Humans; RNA, Messenger; genetics; metabolism; Receptors, Calcitriol; genetics; metabolism
- From: Journal of Southern Medical University 2012;32(1):28-31
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the effect of 25-hydroxyvitamin D(3) on the expression and distribution of vitamin D receptor in normal human bronchial epithelial cells.
METHODSMTT assay was used to assess the viability of human airway epithelial cell line 16HBE following a 24-h exposure to different concentrations of 25-hydroxyvitamin D(3). Real-time quantitative PCR, Western blotting, and immunofluorescence assay were used to observe the expression and distribution of vitamin D receptor in the cells following the exposure.
RESULTSCompared with the control cells, 16HBE cells exposed to different concentrations of 25-hydroxyvitamin D(3) exhibited no significantly increase in the expression or distribution of vitamin D receptor.
CONCLUSIONThe influence of 25-hydroxyvitamin D(3) on bronchial epithelial cells might be independent of the expression and translocation of vitamin D receptor.
