Role of Slit-Robo signaling in the proliferation of human mucoepidermoid carcinoma Mc3 cells.
- Author:
Bing HAN
1
;
Lijing WANG
;
Jing WANG
;
Jie ZHANG
;
Yuan ZHAO
;
Cuiling QI
Author Information
- Publication Type:Journal Article
- MeSH: Carcinoma, Mucoepidermoid; pathology; Cell Line, Tumor; Cell Proliferation; Humans; Intercellular Signaling Peptides and Proteins; metabolism; Nerve Tissue Proteins; metabolism; Proliferating Cell Nuclear Antigen; metabolism; Receptors, Immunologic; metabolism; Salivary Gland Neoplasms; pathology; Signal Transduction
- From: Journal of Southern Medical University 2012;32(1):37-39
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the role of Slit-Robo signaling in the proliferation of human mucoepidermoid carcinoma Mc3 cells.
METHODSWe measured the expressions of Slit2 and Robo1 proteins in human mucoepidermoid carcinoma Mc3 cells using immunohistochemistry and flow cytometry. To test the role of Slit-Robo signaling in the proliferation of the cells, we treated the cells with the monoclonal antibody R5 against Robo1 receptor extracellular domain and observed the changes in the cell proliferation by cell counting and colonogenic assay; the expression of proliferating cell nuclear antigen (PCNA) in the cells following the treatment was measured with flow cytometry.
RESULTSTreatment of Mc3 cells with the monoclonal antibody R5 caused significantly suppressed cell growth and proliferation and obviously lowered the expression of PCNA.
CONCLUSIONSlit-Robo signaling can suppress the proliferation of Mc3 cells possibly by up-regulating the expression of PCNA.
